Effective and simultaneous degradation of many main mycotoxins, using the use of a single or multiple mediators.Toxins 2021, 13,sults indicated that lignin unit-derived natural mediators may possibly be option mediators for mycotoxin degradation by StMCO, when it comes to the economic cost and environmental friendliness. Moreover, the terrific improvement in AFB1 and ZEN degradation in the presence of acetosyringone and ABTS could be attributed for the generation of high possible radicals, aryloxy radicals, and ABTS, respectively [36]. Frequently speaking, these benefits 6 of ten proved that StMCO might be a promising candidate for the effective and simultaneous degradation of several important mycotoxins, with all the use of a single or several mediators.Figure 5. The impact Nitrocefin Epigenetic Reader Domain numerous mediators on the degradation of of AFB (a) and (b) by 0.two U/mL StMCO in 50 mM 50 mM Figure 5. The effect ofof SC-19220 custom synthesis various mediators on the degradationAFB1 (a)1and ZENZEN (b) by 0.two U/mL StMCO in acetate buffer (pH 7.0) containing 1 mg/L AFB1 or AFB1 ormM CuSO4, and 1 mM mediatormediatorat 30 . at 30 C. acetate buffer (pH 7.0) containing 1 mg/L ZEN, five ZEN, 5 mM CuSO4 , and 1 mM for 24 h for 24 hFurthermore, the time courses AFB1 and ZEN degradation by StMCO, within the presFurthermore, the time courses of of AFB1 and ZEN degradation by StMCO, inside the presence of their most efficient mediators, acetosyringone and ABTS, weredetermined. As ence of their most effective mediators, acetosyringone and ABTS, were determined. As shown in Figure six, there was no significant alter in the degradation of AFB1 and ZEN shown in Figure 6, there was no important alter in the degradation of AFB1 and ZEN by StMCO within the absence of mediators right after a 1 h reaction. In contrast, it was notable that by StMCO within the absence of mediators soon after a 1 h reaction. In contrast, it was notable7that Toxins 2021, 13, x FOR PEER Assessment of 11 AFB1and ZEN have been rapidly removed by StMCO in the presence of acetosyringone and AFB1 and ZEN had been quickly removed by StMCO inside the presence of acetosyringone and ABTS, respectively. ABTS, respectively.Figure 6. The time course evaluation of AFB1 (a) and ZEN (b) degradation by 0.2 U/mL StMCO in 50 mM acetate buffer (pH 7.0) containing 1 mg/L AFB1 or ZEN, five mM CuSO4 , and 1degradation by 0.2 U/mLABTS at in 50 mM acetate buffer (pH 7.0) Figure 6. The time course evaluation of AFB1 (a) and ZEN (b) mM acetosyringone or StMCO 30 C. containing 1 mg/L AFB1 or ZEN, 5 mM CuSO4, and 1 mM acetosyringone or ABTS at 30 .two.5. Identification of AFB1 and ZEN Degradation Goods 2.5. Identification of AFB1 and ZEN Degradation Goods Considering that the biological detoxification of mycotoxins was defined because the Considering that the transformation of mycotoxins into significantly less toxic or nontoxic degdegradation or enzymaticbiological detoxification of mycotoxins was defined as the comradation or the degradation solutions of AFB1 and ZEN by less toxic or nontoxic compounds [38], enzymatic transformation of mycotoxins intoStMCO, in the presence in the pounds [38], the degradation identified by UPLC-MS/MS, and their biological toxicities most efficient mediator, wereproducts of AFB1 and ZEN by StMCO, inside the presence from the most effective mediator, had been further elucidated. were identified by UPLC-MS/MS, and their biological toxicities wereAFQ1 was the key degradation item of AFB1 , corresponding to the parent ion additional elucidated. AFQ1 [MH] key degradation item of 311 , corresponding to m/z 283 [.
