Active Recombinant Human IgG1Fc Protein, His-Avi-tagged, Biotinylated

Specification :
| Description : Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases. | Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA against immobilized Recombinant Protein A (0.2 μg/well). The linear range is 0.8-12.5 ng/mL. | Molecular Mass : The protein has a calculated MW of 28.8 kDa. As a result of glycosylation, the protein migrates as 36 kDa under reducing (R) condition, and 60-66 kDa under non-reducing (NR) condition (SDS-PAGE). | Protein length : Pro 100 – Lys 330 (C103S) | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles.This product is stable after storage at:-20°C to -70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.