Active Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His & Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Anti-p40 subunit of IL23（Anti-IL12B） MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 4.9-39 ng/mL (Routinely tested).Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 1.2-19.5 ng/mL (QC tested).Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173ng/ml (Routinely tested).Cell based assay shows that the secretion of IL-17 induced by Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.25-1.40 ng/mL (Routinely tested). | Molecular Mass : 21.9 kDa (IL23A) & 34.7 kDa (IL12B) | Protein length : Arg 20 – Pro 189 (IL23A) & Ile 23 – Ser 328 (IL12B) | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Notes : Please avoid repeated freeze-thaw cycles. | Stability : No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.