Active Recombinant Human IL4 Protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Predicted N Terminal : His 25 | Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. | Bio-activity : Immobilized Human IL-4 R alpha, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human IL-4, His Tag with a linear range of 1.95-15.6 ug/mL. Biotinylated Human IL-4, His Tag, Avi Tag stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ug/mL. | Molecular Mass : The protein has a calculated MW of 17.6 kDa. As a result of glycosylation, the protein migrates as 22 kDa under reducing condition, and 21 kDa under non-reducing condition (SDS-PAGE). | Endotoxin : | Purity : >95% as determined by SDS-PAGE. | Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.