Name :
Recombinant Human IL12B & IL23A protein(Arg20-Pro189&Ile23-Ser328), His-Avi-tagged, Biotinylated
Specification :
| Source : HEK293 | Species : Human | Tag : C-His-Avi | Protein length : Arg20-Pro189&Ile23-Ser328 | Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. | Molecular Mass : The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result. | Endotoxin : Less than 1EU per μg by the LAL method. | Purity : > 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC | Storage : -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. | Reconstitution : Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Information :
| Gene Name : IL12B interleukin 12B [ Homo sapiens (human) ] | Official Symbol : IL12B & IL23A | Synonyms : CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B; IL23A | Gene ID : 3593 | mRNA Refseq : NM_002187 | Protein Refseq : NP_002178 | MIM : 161561 | UniProt ID : P29460
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