Recombinant Human IL1R1 protein, His-tagged

Specification :
| Product overview : Recombinant Human IL1R1 (P14778) aa. (Asp21~Ile109) fused with His tag was produced in E. coli cells. | Description : This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. | Source : E. coli | Species : Human | Tag : His | Form : Freeze-dried powder | Molecular Mass : Predicted Molecular Mass: 11.6kDa; Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions. | Protein length : Asp21~Ile109 | Endotoxin : | Purity : >95% | Characteristic : Isoelectric point is 7.8. | Applications : Cell culture; Activity Assays; In vivo assays. | Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. | Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. | Storage buffer : 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300. | Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex