Ified. Compare active-lever presses of all groups emitted during the last order Cerulenin selfadministration session with each other using a one-way ANOVA. If the F value is not statistically significant (typically understood to be P>0.05), then proceedCurr Protoc Neurosci. Author manuscript; available in PMC 2013 October 01.Beardsley and SheltonPagewith analysis of the data from the final session of extinction as described below. If the F value is statistically significant (typically understood to be P<0.05) then it cannot be concluded that the various groups were trained to similar levels of cocaine self-administration and useful interpretations of the test data are doubtful. PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21113014 36 Compare active-lever presses of all groups emitted during the last extinction session with each other using a one-way ANOVA. If the F value is not statistically significant (typically understood to be P>0.05), then proceed with analysis of the data from the test session as described below. If the F value is statistically significant (typically understood to be P<0.05) then it cannot be concluded that the various groups were extinguished to similar levels of extinction and useful interpretations of the test data are doubtful. Compare active-lever presses of all groups emitted during the test session with each other using a one-way ANOVA. If significant effects are found, conduct Dunnett's Multiple Comparison Tests comparing each test group with the vehicle control group to identify effective treatment effects. Because decisions to proceed with the analysis are made in a step-wise fashion, first determining whether there was effective reinstatement by the vehicle group, then determining whether all groups were trained to a similar level of self-administration, then determining whether all groups were extinguished to a similar level, before the analyses of test session data are performed, a repeated measures ANOVA, in which data from all phases of the experiment are simultaneously included in the analysis, is not conducted. If there is only one treatment group, in addition to the vehicle control group, then t-tests (or non-parametric equivalents) are substituted for the ANOVAs.watermark-text watermark-text watermark-textMaterialsSUPPORT PROTOCOL 1: PREPARATION OF CATHETER LOCK/ ANTIBIOTIC SOLUTIONA specially prepared solution combining the anticoagulant actions of heparin with the viscosity of glycerol and the antibiotic/bacteriostatic actions of the antibiotic Timentin (ticarcillin disodium and clavulanate potassium) is used to fill the jugular catheters between operant test sessions. Use of this solution significantly extends the longevity of the catheter and is highly recommended. The procedure below details preparation of this solution. Note that other antibiotic/bacteriostatic drugs suitable for intravenous administration such as ampicillin/sulbactam may be substituted provided that the amount of pre-flush is properly adjusted to achieve an appropriate dosage.3.1 gram bottle Timentin powder (ticarcillin disodium and clavulanate potassium) 10 ml bottle heparin sodium (1000 units/ml concentration) Glycerol USP (Fisher Scientific or similar) 50 ml sterilized serum vial, butyl rubber stopper and foil retainer in sterilization peel pack 25 mm diameter - 0.2 um pore size sterile syringe filter 60 ml Luer lock sterile disposable syringe 20 ml sterile disposable syringesCurr Protoc Neurosci. Author manuscript; available in PMC 2013 October 01.Beardsley and SheltonPageThree 10 ml.
