Iral ligament, spiral limbus, spiral limbus, and external sulcus cells (Figure 5). DAAM1 is also present in spiral ganglion cells and external sulcus cells (Figure five). DAAM1 can also be present in spiral ganglion cells (Figure 5). Likewise, (Figure five). Likewise, in stria vascularis DAAM1, staining appeared much more evident than CGN and FLNB in stria vascularis DAAM1, staining appeared more evident than CGN and FLNB staining. On P14, staining. On P14, CX26 distributed to the OC, stria vascularis, spiral ligament, spiral limbus, plus the CX26 distributed to the OC, stria vascularis, spiral ligament, spiral limbus, and the spiral ganglion. spiral ganglion. The tectorial membrane is acellular and recognized to yield unspecific staining on account of The tectorial membrane is acellular and recognized to yield unspecific staining as a result of spontaneous spontaneous fluorescence. CX26 and all 3 adaptor proteins seem to be coexpressed inside the OC fluorescence. CX26 and all three adaptor proteins appear to become coexpressed within the OC although OC despite the fact that OC cell identity couldn’t be precisely defined because distinct cell sort markers have not cell identity could not be precisely defined considering that distinct cell type markers haven’t been employed. been employed.Figure four. Indirect immunofluorescence of adult (P60) mouse liver cryosections with antiCx26 Figure 4. Indirect immunofluorescence of adult (P60) mouse liver cryosections with antiCx26 antibody antibody (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is inside a white pseudo (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is in a white pseudo color, colour, based on DAPI staining. The evaluation was performed at confocal microscopy with zsections in accordance with DAPI staining. The evaluation was performed at confocal microscopy with zsections of of 0.five m (LSM880, Carl Zeiss, Oberkochen, Germany). Each image consists on the maximum intensity 0.5 (LSM880, Carl Zeiss, Oberkochen, Germany). Every single image consists with the maximum intensity projection of all zsections Levamlodipine besylate medchemexpress obtained. Arrowheads: signal at the plasma membrane. Arrows: merged projection of all zsections obtained. Arrowheads: signal at the plasma membrane. Arrows: merged signals. Scale bar: ten m. signals. Scale bar: 10 .Int. J. Mol. Sci. 2018, 19,eight ofInt. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW8 ofFigure 5. Indirect immunofluorescence of P14 mouse cochlea cryosections and zoomingin at the Figure 5. Indirect immunofluorescence of P14 mouse cochlea cryosections and zoomingin at the organ organ of Corti (OC) indicated by a rectangle. The sections highlight the spiral ligament (SL), stria of Corti (OC) indicated by a rectangle. The sections highlight the spiral ligament (SL), stria vascularis vascularis (SV), spiral Taurolidine Protocol limbus (SLm), spiral ganglion (SG), external sulcus cells (ES), OC, and tectorial (SV), spiral limbus (SLm), spiral ganglion (SG), external sulcus cells (ES), OC, and tectorial membrane membrane (TM). Labeling by antiCx26 antibody (green) and antibodies for CGN, FLNB, and (TM). Labeling by antiCx26 antibody (green) and antibodies for CGN, FLNB, and DAAM1 (all in DAAM1 (all in red) are presented. DNA is within a white pseudo colour, in line with DAPI staining. The red) are presented. DNA is within a white pseudo color, based on DAPI staining. The analysis was evaluation was performed at confocal microscopy with zsections of 1 m (LSM880, Carl Zeiss, perform.
