Rocess was repeated three times, as well as obtained strong was dried in an oven at 80 C for 24 h. The dried sound was ground for more experiments. two.three. Experimental Procedure The OTC degradation experiments have been initiated by incorporating 1 mM PS to 50 mL of your answer containing twenty OTC and 0.two g/L HWWC. The response was conducted in a shaking incubator at 150 rpm and 25 C. The pH on the resolution was adjusted to 3, 4, six, and eight utilizing 0.1 M NaOH and 0.1 M HCl and analyzed working with a pH meter (Orion Star A211, Thermo, Waltham, MA, USA). To execute the reuse check, the catalyst was magnetically separated immediately after just about every response cycle. two.four. Analytical Method The OTC concentration was measured employing a YL 9100 HPLC technique (Youngin Chromass, Anyang, Korea) which has a YL 9120 UV/Vis detector and YL 9150 autosampler. A YL C18-4D Diversity Library Physicochemical Properties column (four.six mm 150 mm, five ) was used to separate methanol, ACN, and ten mM phosphate buffer (pH of 7) (15:15:70). The mobile phase was isocratically eluted at a flow fee of one.0 mL/min. The column temperature was 35 C, and OTC was detected at 260 nm.Appl. Sci. 2021, 11, x FOR PEER REVIEW3 ofAppl. Sci. 2021, eleven,C18-4D column (four.six mm 150 mm, 5 m) was applied to separate methanol, ACN, and ten mM phosphate buffer (pH of seven) (15:15:70). The mobile phase was isocratically eluted 3at a of ten flow rate of 1.0 mL/min. The column temperature was 35 , and OTC was detected at 260 nm.2.5. Characterization 2.5. Characterization The surface morphology and elemental contents with the surface morphology and elemental contents of the HWWC have been observed working with a observed employing a scanning electron microscope-energy dispersive X-ray spectrometer (SEM-EDS) (JSM-7900F, scanning electron microscope-energy dispersive X-ray spectrometer (SEM-EDS) (JSMJEOL, Tokyo, Japan). The X-ray X-ray diffraction (XRD) pattern of your catalyst was ana7900F, JEOL, Tokyo, Japan). Thediffraction (XRD) pattern of your catalyst was analyzed making use of an XRD system (D/max-2500V, Rigaku, Tokyo, Japan). The Japan). The point (pHpzc lyzed making use of an XRD method (D/max-2500V, Rigaku, Tokyo, stage of zero chargeof zero ) of HWWC was HWWC was established by titration slight modification [27]. HWWC charge (pHpzc) of established by titration technique with technique with slight modification (0.04 g) was (0.04 g) wasin twenty mL of 0.01 M NaNO3 for 24 h. Then the pH of alternative was [27]. HWWC suspended suspended in twenty mL of 0.01 M NaNO 3 for 24 h. Then the pH of adjusted making use of 0.1 M HNO or NaOH answer. To achieve the equilibrium, the option was resolution was adjusted using30.1 M HNO3 or NaOH solution. To reach the equilibrium, the agitated for 1 h, then the pHinitial was measured. After measuring the pHinitial 0.six g of option was agitated for one h, then the pHinitial was measured. Right after measuring the ,pHinitial, NaNO was extra additional to your suspension. After 3 h, the the alternative remedy was 0.six g of 3NaNO3 was for the suspension. Following 3 h, the Benidipine Membrane Transporter/Ion Channel pHfinal ofpH ultimate in the was measured. The pHpzc value pzc worth was determined as pH (pHfinal Hinitial)0 when plotting pH measured. The pH was determined as pH (pHfinal Hinitial ) was was 0 when plotting against pH pH As shown in in Figure pHpzc of HWWC was seven.four. The magnetic property pH againstfinal .last. As shownFigure 1a, 1a, pHpzc of HWWC was seven.4. The magnetic propof of HWWC was measured working with vibrating sample magnetometer (VSM) (Model 7404, ertyHWWC was measured using vibrating sample magnetometer (VSM) (Model 7404, Lake shore cryotronics, Westerville, Lake shore c.
