Winooski, VT, USA).Antioxidants 2021, 10,3 ofFor the lipidomics analysis, the following reagents
Winooski, VT, USA).Antioxidants 2021, 10,3 ofFor the lipidomics analysis, the following reagents (all LC S grade, bought from Merck (Merck KGaA, Darmstadt, Germany) have been employed: isopropanol, methanol, and water. Lastly, the phase modifiers, namely formic acid and ammonium formate, have been purchased from Merck (Merck KGaA, Darmstadt, Germany). two.2. Pumpkin Samples and Preparation Twenty studied accessions, reported in Figures 1 and two, were grown and harvested in Ba ki Petrovac, North Serbia. Pumpkins and squashes had been grown under regular c production conditions for Serbia, except for the distances among the rows, which were five m. Mineral fertilizer was added towards the soil, there was no irrigation considering that there was sufficient moisture within the soil, and pesticides have been not applied because there had been no considerable illnesses. To stop and shield against weeds, inter-row cultivation was performed on two occasions and within the rows, and weeds have been destroyed manually. Sowing was performed in early May well, and fruits had been harvested from the finish of September to late October 2018. Pumpkin pulp samples were taken from five fruits within the exact same plot (plot number = sample number). The fresh pumpkin samples (50 g every) were then freeze-dried at 0 C on a Christ Alpha 1-2 LD Freeze Dryer (Switzerland) for 48 h.tioxidants 2021, 10,4 ofFigure 1. Pumpkins collected or bred in Serbia: photos, sample number (in bold) associated with plot quantity in which every CD70 Proteins MedChemExpress selection collected and absorbance spectra (blue line = 1st number (in bold) = 2nd extraction. See Section two.3). Figure 1. Pumpkins was grown,or bred in Serbia: photos, sampleextraction; orange line linked with plot number in which The absorbance spectra cover the absorbance (blue line = from 0 to 1 (increments of 0.1) 2nd from 250 to see Section two.3.). every assortment was grown, and absorbance spectra worth ranging 1st extraction; orange line = and extraction. 600 nm (increments of 50 nm). the absorbance value ranging from 0 in Figure S1 (Supplementary material) and weight, The absorbance spectra coverPumpkin calibres are a lot more clearly visualizedto 1 (increments of 0.1) and from 250 to 600 nm (increlength, and diameter are reported more S3 (Supplementary material). ments of 50 nm). Pumpkin calibres arein Tableclearly visualized in Figure S1 (Supplementary material) and weight, length,and diameter are reported in Table S3 (Supplementary material).Figure 1. Pumpkins collected or bred in Serbia: images, sample quantity (in bold) linked with plot numbe each range was grown, and absorbance spectra (blue line = 1st extraction; orange line = 2nd extraction. See Se The absorbance spectra cover the absorbance worth ranging from 0 to 1 (increments of 0.1) and from 250 to 600 ments of 50 Antioxidants 2021, ten, 1580nm). Pumpkin calibres are more clearly visualized in Figure S1 (Supplementary material)16 4 of and weig and diameter are reported in Table S3 (Supplementary material).Figure two. Pumpkins Figure 2. Pumpkins not Serbia: pictures, sample number (in bold)(in bold) connected plot numbe not Aminopeptidase N/CD13 Proteins Biological Activity initially from initially from Serbia: pictures, sample quantity linked with with plot geographical every single variety was grown, and absorbance spectra (blue line = 1st extraction; o every assortment was grown,quantity in whichorigin (when identified),geographical origin (when known), and absorbance spectra two.3.). The absorbance orange line = 2nd extraction. See values ranging from 0 to = 2nd extraction. See Section(blue line = 1st extraction; s.
