Active Recombinant Human CD8A protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human CD8 alpha, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 1-2 ng/mL.Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind Human CD8 alpha, His Tag with a linear range of 0.6-10 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 19.5 kDa. The protein migrates as 26-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Ser22-Asp182 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. | Publication : Aptamer-Containing Surfaces for Selective Capture of CD4 Expressing Cells (2012)