Active Recombinant Human CD80 Protein, Fc & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : Fc & Avi | Predicted N Terminal : Val 35 | Form : Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.6-9.8 ng/mL. | Molecular Mass : Recombinant Human CD80 Protein has a calculated MW of 52.3 kDa. As a result of glycosylation, The protein migrates as 65-95 kDa under reducing (R) condition and 130-160 kDa under non-reducing (NR) condition on SDS-PAGE gel. | Protein length : Val 35 – Asn 242 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.