Recombinant Human CTSB protein, His-tagged

Specification :
| Description : Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B/CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B/ APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, Cathepsin B/CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of Cathepsin B has been associated with esophageal adenocarcinoma and other tumors. | Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose. | Molecular Mass : The protein has a calculated MW of 36.7 kDa (pro-form) and 29.0 kDa (mature-form). Due to glycosylation, The reducing (R) protein migrates as 42-50 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band. | Protein length : 322 | Endotoxin : Less than 1.0 EU per ug by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.Please avoid repeated freeze-thaw cycles.This product is stable after storage at:-20 centigrade to -70 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.