Name :
Active Recombinant Human EGFR protein, His-Avi-tagged, Biotinylated
Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. | Bio-activity : Immobilized Biotinylated Human EGFRvIII, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-EGFRvIII Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL.Immobilized CetuxiMab at 1 μg/mL (100 μL/well) can bind Biotinylated Human EGFRvIII, His,Avitag with a linear range of 0.2-2 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 42.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Leu 25- Ser 378 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Information :
| Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens ] | Official Symbol : EGFR | Synonyms : EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61; | Gene ID : 1956 | mRNA Refseq : NM_005228 | Protein Refseq : NP_005219 | MIM : 131550 | UniProt ID : P00533
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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