Active Recombinant Human FCGR1A Protein, Avi/His-tagged, biotinylated

Specification :
| Description : Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. | Source : HEK293 | Species : Human | Tag : Avi/His | Predicted N Terminal : Gln 16 | Form : Lyophilized from sterile PBS, pH 7.41. Normally 5 % – 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements. | Bio-activity : Immobilized recombinant CD64-AVI-His at 10 μg/ml (100 μl/well) can human IgG1 with a linear range of 1.56-50 ng/ml. Labeling ratio of biotin to protein: 1.09. | Molecular Mass : The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa. | Protein length : Met 1-Thr 287 | Endotoxin : | Purity : > 90 % as determined by SDS-PAGE | Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade | Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex. | Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.