The cells had been washed with ice-chilly PBS and lysed in immunoprecipitation buffer (50 mM Tris pH 8., one hundred fifty mM NaCl, 1.% Nonidet P-forty, .five% deoxycholate, .one% SDS, five mM EDTA and protease and phosphatase inhibitors)
All tissue culture reagents ended up acquired from Invitrogen (Gaithersburg, MD). Amaxa mobile transfection...