Product: AP05762PU-NALOX5 / LOG5 pSer523 antibody
Quantity: 0.1 ml
Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase
Presentation: Purified
Clonality: Polyclonal
Host: Rabbit
Isotype: IgG
CAS NO: 51781-21-6 Product: Carteolol (hydrochloride)
Shipping to: Worldwide
Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within Human 5-lipoxygenase which includes phosphorylated Ser523.GeneID:240
Application: Western Blot: 1/1000. Detects a doublet of approximately 80kDa in Rat cortex lysate. For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking
Background: 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5LOactivating protein as cofactors
Concentration:
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 10mM HEPES pH7.5, 150mM NaCl containing 0.09% Sodium Azide, 0.01% BSA, 50% Glycerol
Preservatives:
State: Liquid purified IgG fractionPurified
Specifictiy: This antibody specifically recognizes Human 5-lipoxygenase (5-LO, also known as arachidonate 5-lipoxygenase) when phosphorylated at Ser523.
