Product: AP23298PU-NAlkaline phosphatase / ALPL (N-term) antibody
Quantity: 0.1 mg
Synonyms: AP-TNAP, Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, TNSALP, tissue-nonspecific isozyme
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype: IgG
CAS NO: 141400-58-0 Product: PX-12
Shipping to: Worldwide
Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of Human ALPL (21-35)AA Sequence:EKEKDPKYWRDQAQEGeneID:249Remarks:Sequence is different from the related rat and mouse sequence by two amino acids.
Application: Western blot: At 1-2μg/ml with the appropriate system to detect ALPL in cells and tissues.The detection limit for ALPL is approximately 2.5 ng/lane under non-reducing and reducing conditions.Immunohistochemistry on Paraffin Sections: At 1-2 μg/ml to detec
Background: Alkaline phosphatase (ALPL) removes phosphate groups from the 5 end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Ti
Concentration:
Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal and 0.05mg Sodium Azide
Preservatives:
State: Lyophilized purified Ig fractionAff – Purified
Specifictiy: This antibody detects Alkaline phosphatase / ALPL at N-term.No cross reactivity with other proteins.
