Product: TA319277AKT1/AKT2/AKT3 (C-term) antibody
Quantity: 0.2 ml
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation: Serum
Clonality: Polyclonal
Host: Rabbit
Isotype:
CAS NO: 1781882-65-2 Product: Pyridostatin (hydrochloride)
Shipping to: Europe, USA/Canada
Immunogen: Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.AA Sequence:C-R-PH-F-P-Q-F-S-Y
Application: ELISA: 1/2,000-1/10,000.Immunoprecipitation.Transfected/Primary cell culture.Western Blot (1/500-1/2,000): Detect a single band of the expected apparent molecular weight.Immunofluorescence using paraformaldehyde-fixed primary cardiomyocyte cultures (1/100
Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transdu
Concentration: 75 mg/ml (by refractometry)
Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% Sodium Azide
Preservatives:
State: Liquid whole serum.Serum
Specifictiy: This antiserum reacts with the AKT.
